LUMI-PCR: an Illumina platform ligation-mediated PCR protocol for integration site cloning, provides molecular quantitation of integration sites.

LUMI-PCR: an Illumina platform ligation-mediated PCR protocol for integration site cloning, provides molecular quantitation of integration sites.

Ligation-mediated PCR protocols have various makes use of together with the identification of integration websites of insertional mutagens, integrating vectors and naturally occurring cellular genetic parts. For approaches that make use of NGS sequencing, the relative abundance of integrations inside a posh combination is usually decided by using learn counts or distinctive fragment lengths from …

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