Alloplasmic strains are an acceptable mannequin for finding out molecular coevolution and interrelations between genetic programs of plant cells. Complete chloroplast (cp) and mitochondrial (mt) genome sequences had been obtained by the MiSeq System (Illumina). Organelle DNA samples had been ready from a set of 12 alloplasmic barley strains with completely different cytoplasms of Hordeum vulgare ssp. spontaneum and H. vulgare ssp. vulgare, in addition to from their paternal varieties. A bioinformatic strategy for evaluation of NGS information obtained on an organellar DNA combine has been developed and verified.
A comparative research of Hordeum organelle genomes’ variability and disposition of polymorphic loci was performed. Eight forms of chloroplast DNA and 5 forms of mitochondrial DNA had been distinguished for the barley pattern set examined. These outcomes had been in contrast with the earlier information of a restriction fragment size polymorphism (RFLP) research of organelle DNAs for a similar materials. Previously established information a few discipline analysis of alloplasmic barley strains had been revised within the gentle of details about organelle genomes gained after NGS. Completely 17 polymorphic loci had been discovered at exons of chloroplast genomes.
Seven of the SNPs had been situated within the genes of the Ndh complicated. The nonsynonymous adjustments of nucleotides had been detected within the matK, rpoC1, ndhK, ndhG and infA genes. A few of the SNPs detected are very comparable in codon place and in the kind of amino acid substitution to the locations the place RNA modifying can happen. Thus, these outcomes define new views for the long run research of nuclear-cytoplasmic interactions in alloplasmic strains. Roughly 10%, 27%, and 63% of the 540 samples contained < 1500 GE, a variety of 1500-3000 GE, and > 3000 GE, which corresponded to a maximal assay sensitivity of two.0%, 0.5-0.1%, and 0.1-0.05% mutant allelic fraction, respectively.
The sensitivity and specificity had been measured for this BEAMing assay. The variety of mutant beads and mutant allelic fraction had been measured for every EGFR alteration and the extent of detection was established at 0.1% for a median of 2861 genome equal (GE) in every response utilizing HD780 horizon management DNA, in addition to by an inner high quality reference commonplace. In a routine hospital setting, 11.4% of non-small cell lung most cancers tumors had been constructive at prognosis for EGFR alterations, whereas 43.7% samples harbored EGFR mutations at development, amongst which 40.3% expressed EGFR resistance mutations after first-line tyrosine kinase inhibitor remedy with first- and second-generation medication.
Molecular subtype prognosis of endometrial carcinoma: comparability of NGS panel and ProMisE classifier
The Most cancers Genome Atlas-based molecular classification of endometrial carcinoma (EC) has the potential to higher determine these sufferers whose illness is prone to behave in another way than predicted when utilizing conventional threat stratification, nonetheless, the optimum strategy to molecular subtype task in routine observe stays undetermined. The goal of this research was to check the outcomes of two completely different broadly obtainable approaches to prognosis of EC molecular subtype.
EC from 60 sufferers, had been molecularly subclassified utilizing two completely different strategies; by performing the FoundationOne CDx Subsequent Technology Sequencing (NGS) panel and utilizing the Proactive Molecular Threat Classifier for Endometrial Most cancers (ProMisE) classifier and performing immunohistochemical stainings for MMR proteins and p53. MSI standing may very well be decided based mostly on the NGS panel ends in 53 of 60 tumors, so ProMisE and NGS molecular subtype task may very well be straight in contrast for these 53 tumors. Molecular subtype prognosis based mostly on NGS and ProMisE was in settlement for 52 of 53 tumors. One tumor was microsatellite steady (MSS) however confirmed lack of MLH1 and PMS2 expression.
Molecular subtype prognosis of EC based mostly on NGS panel sequencing of formalin-fixed paraffin-embedded endometrial carcinomas and based mostly totally on immunostaining (ProMisE) yield equivalent ends in 98.1% (52/53, kappa – 0.97) of circumstances. Whereas outcomes are comparable utilizing these two approaches, every has benefits and drawbacks that may affect the selection of methodology for use in scientific observe. POLE mutation standing was in each settings derived from FoundationOne outcomes. Molecular classification based mostly on ProMisE was profitable for all 60 tumors.

[NGS sequencing in barley breeding and genetic studies]
Barley (Hordeum vulgare L.) is the some of the necessary cereal species used as meals and feed crops, in addition to for malting and alcohol manufacturing. On the finish of the final century, conventional breeding methods had been complemented by means of DNA markers. Molecular markers have additionally been used extensively for molecular genetic mapping and QTL evaluation. In 2012, the barley genome sequencing was accomplished, which supplied a broad vary of recent alternatives – from a extra environment friendly seek for candidate genes controlling economically necessary traits to genomic choice.
The evaluation summarizes the outcomes of the research carried out after barley genome sequencing, which found new areas of barley genetics and breeding with excessive throughput screening and genotyping strategies. Throughout this era, intensive research geared toward identification of barley genomic loci related to economically necessary traits have been carried out; on-line databases and instruments for working with barley genomic information and their deposition have appeared and are being replenished. Lately, GWAS evaluation has been used for large-scale phenotypegenotype affiliation research, which has been broadly utilized in barley since 2010 because of the developed SNP-arrays, in addition to genotyping strategies based mostly on direct NGS sequencing of chosen fractions of the genome.
Standard UV Quartz Cell with Teflon Stopper |
SQ014 |
Bio Basic |
1 UNIT |
EUR 147.24 |
|
transilluminator led 20x12 cm + hood |
BLOOK |
Consort |
ea |
EUR 580 |
Semi-Micro UV Quartz Cell With Frosted Walls and with Teflon Stopper |
SQ294 |
Bio Basic |
1 UNIT |
EUR 171.19 |
|
Micro UV Quartz Cell With Lid, Inside Width: 2mm, 0.7ml |
SQ244 |
Bio Basic |
1 UNIT |
EUR 123.3 |
|
Micro UV Quartz Cell With Lid, Inside Width: 4mm, 1.4ml |
SQ264 |
Bio Basic |
1 UNIT |
EUR 123.3 |
|
Micro UV Quartz Cell With Lid, Inside Width: 5mm, 1.7ml |
SQ274 |
Bio Basic |
1 UNIT |
EUR 123.3 |
|
UV TRANSMITTING TRAY |
GD-GCS |
CORNING |
1/pk |
EUR 208 |
Description: Lab Equipment; Axygen Branded EQ |
Fluorescent UV Particles |
FP-15040-2 |
Spherotech |
2 mL |
EUR 283 |
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
uv protected glasses |
VLLP70 |
Consort |
ea |
EUR 99 |
Semi-Micro UV Quartz Cell With Black Walls And With Lid, Inside Width: 4mm, 1.4ml |
SQ024 |
Bio Basic |
1 UNIT |
EUR 123.3 |
|
Semi-Micro UV Quartz Cell With Black Walls And With Lid, Inside Width: 5mm, 1.7ml |
SQ144 |
Bio Basic |
1 UNIT |
EUR 123.3 |
|
Semi-Micro UV Glass Cell With Black Walls And With Lid, Inside Width: 4mm, 1.4ml |
S024-G |
Bio Basic |
1 UNIT |
EUR 104.15 |
|
Semi-Micro UV Glass Cell With Black Walls And With Lid, Inside Width: 5mm, 1.7ml |
S144-G |
Bio Basic |
1 UNIT |
EUR 104.15 |
|
SmartDoc UV Blocking Mat |
E5000-MAT |
BenchMark |
1 PC |
EUR 100.67 |
|
uv lamp 1x15w, 254nm |
VL115-C |
Consort |
ea |
EUR 656 |
uv lamp 1x15w, 365nm |
VL115-L |
Consort |
ea |
EUR 656 |
uv lamp 1x15w, 312nm |
VL115-M |
Consort |
ea |
EUR 656 |
uv lamp 2x15w, 254nm |
VL215-C |
Consort |
ea |
EUR 792 |
uv lamp 2x15w, 365nm |
VL215-L |
Consort |
ea |
EUR 792 |
uv lamp 2x15w, 312nm |
VL215-M |
Consort |
ea |
EUR 817 |
uv lamp 1x6w, 254nm |
VL6-C |
Consort |
ea |
EUR 521 |
uv lamp 1x6w, 365nm |
VL6-L |
Consort |
ea |
EUR 521 |
conversion screen uv/blue |
VLFC26-BLUE |
Consort |
ea |
EUR 606 |
conversion screen uv/wl |
VLFC26-WL |
Consort |
ea |
EUR 606 |
uv protected face shield |
VLMP80 |
Consort |
ea |
EUR 183 |
uv protected face shield |
VLMP800 |
Consort |
ea |
EUR 200 |
UV Carboxyl Particle Array Kit |
UVCPAK--5042-14K |
Spherotech |
14X1 mL |
EUR 1278 |
Description: UltraRainbow Fluorescent Particlesare calibration particles that help you to determine if a flow cytometer is properly aligned and has a clean flow cell with no fluidic blockage. Using SPHERO? Alignment Particles, the coefficients of variation (CVs), peak channels, and histogram distributions can be measured to determine the alignment and functionality of the flow cytometer. |
uv lamp 2x15w, 254/365nm |
VL215-LC |
Consort |
ea |
EUR 817 |
uv lamp 2x15w, 312/365nm |
VL215-LM |
Consort |
ea |
EUR 817 |
uv lamp 2x15w, 254/312nm |
VL215-MC |
Consort |
ea |
EUR 859 |
uv lamp 2x6w, 254/365nm |
VL6-LC |
Consort |
ea |
EUR 538 |
starter uv tube 4w-20w |
VLST151 |
Consort |
ea |
EUR 56 |
uv table 8w 254nm 15x15 cm |
VLECX-15C |
Consort |
ea |
EUR 1028 |
uv table 8w 312nm 15x15 cm |
VLECX-15M |
Consort |
ea |
EUR 1028 |
uv table blue led 20x20 cm |
VLECX-20BLUE |
Consort |
ea |
EUR 1535 |
uv table 8w 254nm 20x20 cm |
VLECX-20C |
Consort |
ea |
EUR 1113 |
uv table 8w 365nm 20x20 cm |
VLECX-20L |
Consort |
ea |
EUR 1113 |
uv table 8w 312nm 20x20 cm |
VLECX-20M |
Consort |
ea |
EUR 1163 |
uv table 8w, 254nm 21x26 cm |
VLECX-26C |
Consort |
ea |
EUR 1197 |
uv table 8w, 312nm 21x26 cm |
VLECX-26M |
Consort |
ea |
EUR 1248 |
uv tube 15 w, 254 nm |
VLT15-C |
Consort |
ea |
EUR 56 |
uv tube 15 w, 365 nm |
VLT15-L |
Consort |
ea |
EUR 56 |
uv tube 15 w, 312 nm |
VLT15-M |
Consort |
ea |
EUR 82 |
uv tube 4 w, 254 nm |
VLT4-C |
Consort |
ea |
EUR 56 |
uv tube 4 w, 365 nm |
VLT4-L |
Consort |
ea |
EUR 56 |
uv tube 6 w, 254 nm |
VLT6-C |
Consort |
ea |
EUR 56 |
uv tube 6 w, 365 nm |
VLT6-L |
Consort |
ea |
EUR 56 |
uv tube 8 w, 254 nm |
VLT8-C |
Consort |
ea |
EUR 56 |
uv tube 8 w, 365 nm |
VLT8-L |
Consort |
ea |
EUR 56 |
uv tube 8 w, 312 nm |
VLT8-M |
Consort |
ea |
EUR 82 |
Standard UV Quartz Cell With Lid, 45 X 12.5 X 7.5 mm, Inside Width: 10mm, 1.7ml |
SQ003 |
Bio Basic |
1 UNIT |
EUR 93.5 |
|
Standard UV Quartz Cell With Lid, 45 X 12.5 X 12.5 mm, Inside Width: 10mm, 3.5ml |
SQ004 |
Bio Basic |
1 UNIT |
EUR 100.46 |
|
Standard UV Quartz Cell With Lid, 45 X 12.5 X 22.5 mm, Inside Width: 10mm, 7.0ml |
SQ005 |
Bio Basic |
1 UNIT |
EUR 128.09 |
|
AXYGEN® GEL TRAY 10 X 10 CM FOR USE WITH 10 CM GEL BOX, UV TRANSPARENT |
HGB10-10-GT |
CORNING |
1/pk |
EUR 74 |
Description: Lab Equipment; Axygen Branded EQ |
AXYGEN® GEL TRAY 15 X 10 CM FOR USE WITH 15 CM GEL BOX, UV TRANSPARENT |
HGB15-10-GT |
CORNING |
1/pk |
EUR 79 |
Description: Lab Equipment; Axygen Branded EQ |
AXYGEN® GEL TRAY 15 X 15 CM FOR USE WITH 15 CM GEL BOX, UV TRANSPARENT |
HGB15-15-GT |
CORNING |
1/pk |
EUR 79 |
Description: Lab Equipment; Axygen Branded EQ |
AXYGEN® GEL TRAY 20 X 20 CM FOR USE WITH 20 CM GEL BOX, UV TRANSPARENT |
HGB20-20-GT |
CORNING |
1/pk |
EUR 97 |
Description: Lab Equipment; Axygen Branded EQ |
AXYGEN® GEL TRAY 7 X 10 CM FOR USE WITH 7 CM GEL BOX, UV TRANSPARENT |
HGB7-10-GT |
CORNING |
1/pk |
EUR 68 |
Description: Lab Equipment; Axygen Branded EQ |
AXYGEN® GEL TRAY 7 X 7 CM FOR USE WITH 7 CM GEL BOX, UV TRANSPARENT |
HGB7-7-GT |
CORNING |
1/pk |
EUR 61 |
Description: Lab Equipment; Axygen Branded EQ |
Bacillus cereus UV DNA damage endonuclease (uvsE) |
1-CSB-EP467668BQP |
Cusabio |
-
EUR 611.00
-
EUR 309.00
-
EUR 1827.00
-
EUR 939.00
-
EUR 1218.00
-
EUR 397.00
|
-
100ug
-
10ug
-
1MG
-
200ug
-
500ug
-
50ug
|
|
Description: Recombinant Bacillus cereus UV DNA damage endonuclease(uvsE) expressed in E.coli |
WSE-5300 Printgraph CMOS I 6M UV |
2305300 |
Atto |
1unit |
EUR 8510 |
Description: I ncl udes 312 nm U V t r ansi l l umi nat or , Whi t e L EDl i ght pl at e CE marked |
PLT,96WL,UV,NT,NS,25/50 |
3635 |
CORNING |
25/pk |
EUR 545 |
Description: Genomics; Genomic Plates |
Uv Radiation Resistance Associated Gene (UVRAG) Antibody |
20-abx116558 |
Abbexa |
|
|
|
uv table 8w 365/254nm 20x20 cm |
VLTCP-20LC |
Consort |
ea |
EUR 1282 |
uv table 8w 365/312nm 20x20 cm |
VLTCP-20LM |
Consort |
ea |
EUR 1282 |
uv table 8w 312/254nm 20x20 cm |
VLTCP-20MC |
Consort |
ea |
EUR 1282 |
uv table 2x8w, 365/254nm 21x26 cm |
VLTCP-26LC |
Consort |
ea |
EUR 1620 |
uv table 2x8w, 365/312nm 21x26 cm |
VLTCP-26LM |
Consort |
ea |
EUR 1620 |
uv table 2x8w, 312/254nm 21x26 cm |
VLTCP-26MC |
Consort |
ea |
EUR 1620 |
UV excision repair protein RAD23 homolog A Antibody |
20-abx110497 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
-
100 ug
-
1 mg
-
200 ug
-
20 ug
-
50 ug
|
|
UV Radiation Resistance-Associated Gene Protein (UVRAG) Antibody |
20-abx006162 |
Abbexa |
-
EUR 411.00
-
EUR 592.00
-
EUR 182.00
-
EUR 314.00
|
-
100 ul
-
200 ul
-
20 ul
-
50 ul
|
|
UV Radiation Resistance-Associated Gene Protein (UVRAG) Antibody |
abx030355-400ul |
Abbexa |
400 ul |
EUR 523 |
|
UV Radiation Resistance-Associated Gene Protein (UVRAG) Antibody |
abx030355-80l |
Abbexa |
80 µl |
EUR 286 |
|
UV Radiation Resistance-Associated Gene Protein (UVRAG) Antibody |
abx030356-400ul |
Abbexa |
400 ul |
EUR 523 |
|
UV Radiation Resistance-Associated Gene Protein (UVRAG) Antibody |
abx030356-80l |
Abbexa |
80 µl |
EUR 286 |
|
UV Radiation Resistance-Associated Gene Protein (UVRAG) Antibody |
abx030357-400ul |
Abbexa |
400 ul |
EUR 523 |
|
UV Radiation Resistance-Associated Gene Protein (UVRAG) Antibody |
abx030357-80l |
Abbexa |
80 µl |
EUR 286 |
|
UV Radiation Resistance-Associated Gene Protein (UVRAG) Antibody |
abx030358-400ul |
Abbexa |
400 ul |
EUR 523 |
|
UV Radiation Resistance-Associated Gene Protein (UVRAG) Antibody |
abx030358-80l |
Abbexa |
80 µl |
EUR 286 |
|
UV Radiation Resistance-Associated Gene Protein (UVRAG) Antibody |
abx448488-100ug |
Abbexa |
100 ug |
EUR 523 |
|
UV Radiation Resistance-Associated Gene Protein (UVRAG) Antibody |
abx448489-100ug |
Abbexa |
100 ug |
EUR 523 |
|
UV Radiation Resistance-Associated Gene Protein (UVRAG) Antibody |
20-abx313923 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
-
100 ug
-
1 mg
-
200 ug
-
20 ug
-
50 ug
|
|
UV Radiation Resistance-Associated Gene Protein (UVRAG) Antibody |
abx239352-100ug |
Abbexa |
100 ug |
EUR 509 |
|
BioWand Personal UV Sanitizer, 265-280nm, 1/ea |
U1001-W |
MTC Bio |
1/pack |
EUR 96.58 |
Description: BioWand Personal UV Sanitizer 265-280nm |
uv table 8w 254nm/wl 2x(20x20) cm |
VLTFP-CWL |
Consort |
ea |
EUR 1535 |
uv table 8w 365nm/wl 2x(20x20) cm |
VLTFP-LWL |
Consort |
ea |
EUR 1535 |
uv table 8w 312nm/wl 2x(20x20) cm |
VLTFP-MWL |
Consort |
ea |
EUR 1535 |
One-Step Lumitein UV Protein Gel Stain, 1X |
21005-1L |
Biotium |
1L |
EUR 222 |
Description: Minimum order quantity: 1 unit of 1L |
One-Step Lumitein UV Protein Gel Stain, 1X |
21005-4L |
Biotium |
4L |
EUR 567 |
Description: Minimum order quantity: 1 unit of 4L |
One-Step Lumitein UV Protein Gel Stain, 1X |
21005-F |
Biotium |
25mL |
EUR 50 |
Description: Minimum order quantity: 1 unit of 25mL |
Replacement power supply for nUVaClean UV Pipette Carousel |
P5590-PS |
MTC Bio |
1/pack |
EUR 76.33 |
Description: Replacement power supply for nUVaClean UV Pipette Carousel |
PhosphoWorks™ Colorimetric MESG Phosphate Assay Kit *UV absorption* |
21659 |
AAT Bioquest |
200 Tests |
EUR 306 |
|
Human UV excision repair protein RAD23 homolog A (RAD23A) |
1-CSB-YP019259HU |
Cusabio |
-
EUR 430.00
-
EUR 234.00
-
EUR 1508.00
-
EUR 642.00
-
EUR 1009.00
-
EUR 291.00
|
-
100ug
-
10ug
-
1MG
-
200ug
-
500ug
-
50ug
|
|
Description: Recombinant Human UV excision repair protein RAD23 homolog A(RAD23A) expressed in Yeast |
Human UV excision repair protein RAD23 homolog A (RAD23A) |
1-CSB-EP019259HU |
Cusabio |
-
EUR 380.00
-
EUR 214.00
-
EUR 1309.00
-
EUR 560.00
-
EUR 873.00
-
EUR 262.00
|
-
100ug
-
10ug
-
1MG
-
200ug
-
500ug
-
50ug
|
|
Description: Recombinant Human UV excision repair protein RAD23 homolog A(RAD23A) expressed in E.coli |
UV excision repair protein RAD23 homolog A Antibody (Biotin) |
20-abx106211 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
-
100 ug
-
1 mg
-
200 ug
-
20 ug
-
50 ug
|
|
UV excision repair protein RAD23 homolog A Antibody (FITC) |
20-abx107625 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
-
100 ug
-
1 mg
-
200 ug
-
20 ug
-
50 ug
|
|
UV excision repair protein RAD23 homolog A Antibody (HRP) |
20-abx109043 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
-
100 ug
-
1 mg
-
200 ug
-
20 ug
-
50 ug
|
|
Human UV-Stimulated Scaffold Protein A (UVSSA) ELISA Kit |
abx388132-96tests |
Abbexa |
96 tests |
EUR 911 |
|
UV Radiation Resistance-Associated Gene Protein (UVRAG) Antibody (ALP) |
abx446398-100ug |
Abbexa |
100 ug |
EUR 578 |
|
UV Radiation Resistance-Associated Gene Protein (UVRAG) Antibody (ALP) |
abx446399-100ug |
Abbexa |
100 ug |
EUR 578 |
|
UV Radiation Resistance-Associated Gene Protein (UVRAG) Antibody (APC) |
abx446400-100ug |
Abbexa |
100 ug |
EUR 578 |
|
UV Radiation Resistance-Associated Gene Protein (UVRAG) Antibody (APC) |
abx446401-100ug |
Abbexa |
100 ug |
EUR 578 |
|
UV Radiation Resistance-Associated Gene Protein (UVRAG) Antibody (Biotin) |
abx446402-100ug |
Abbexa |
100 ug |
EUR 578 |
|
UV Radiation Resistance-Associated Gene Protein (UVRAG) Antibody (Biotin) |
abx446403-100ug |
Abbexa |
100 ug |
EUR 578 |
|