The emergence of Mycobacterium tuberculosis with complex drug resistance profiles necessitates a rapid and comprehensive drug susceptibility test for guidance of patient treatment. We developed two targeted-sequencing workflows based on Illumina MiSeq and Nanopore MinION for the prediction of drug resistance in M.
tuberculosis toward 12 antibiotics.A total of 163 M. tuberculosis isolates collected from Hong Kong and Ethiopia were subjected to a multiplex PCR for simultaneous amplification of 19 drug resistance-associated genetic regions.
The amplicons were then barcoded and sequenced in parallel on MiSeq and MinION in respective batch sizes of 24 and 12 samples. A web-based bioinformatics pipeline, BacterioChek-TB, was developed to translate the raw datasets into clinician-friendly reports.Both platforms successfully sequenced all samples with mean read depths of 1,127× and 1,649×, respectively.
The variant calling by MiSeq and MinION could achieve 100% agreement if variants with an allele frequency of <40% reported by MinION were excluded. Both workflows achieved a mean clinical sensitivity of 94.8% and clinical specificity of 98.0% when compared with phenotypic drug susceptibility test (pDST).
Turnaround times for the MiSeq and MinION workflows were 38 and 15 h, facilitating the delivery of treatment guidance at least 17-18 days earlier than pDST, respectively.
 Protein) Recombinant Human PF-4 (CXCL4) Protein |
|
PROTP02776-4 |
BosterBio |
20ug |
EUR 317 |
|
Description: PF-4 is a CXC chemokine that is expressed in megakaryocytes and stored in the α-granules of platelets. PF-4 is chemotactic towards neutrophils and monocytes and has been shown to inhibit angiogenesis. Recombinant human PF-4 is a 7.8 kDa protein containing 70 amino acid residues, including the four highly conserved residues present in CXC chemokines. |
 Mouse FibrOut 4, for brain, neural |
|
4-20507 |
CHI Scientific |
1 ml |
Ask for price |
Mouse FibrOut 4, for brain, neural |
|
4-20508 |
CHI Scientific |
5 x 1 ml |
Ask for price |
 Rat FibrOut 4, for brain, neural |
|
4-20533 |
CHI Scientific |
1 ml |
Ask for price |
Rat FibrOut 4, for brain, neural |
|
4-20534 |
CHI Scientific |
5 x 1 ml |
Ask for price |
 Human FibrOut 4, for brain, neural |
|
4-21552 |
CHI Scientific |
1 ml |
Ask for price |
Human FibrOut 4, for brain, neural |
|
4-21553 |
CHI Scientific |
5 x 1 ml |
Ask for price |
 DIGITAL SHAKER FOR 4 MICROPLATES, 120V |
|
6780-4 |
CORNING |
1/pk |
EUR 798 |
|
Description: Lab Equipment; Shakers & Mixers |
 Recombinant Human 4-1BB Receptor Protein |
|
PROTQ07011-4 |
BosterBio |
20ug |
EUR 317 |
|
Description: 4-1BB Receptor, a member of the TNF superfamily of receptors, is mainly expressed on the surface of a variety of T cells, but also found in B cells, monocytes, and various transformed cell lines. 4-1BB Receptor binds to 4-1BBL to provide a co-stimulatory signal for T lymphocytes. Signaling by 4-1BB Receptor has been implicated in the antigen-presentation process and generation of cytotoxic T cells. The human 4-1BB Receptor gene codes for a 255 amino acid type I transmembrane protein containing a 17 amino acid N-terminal signal sequence, a 169 amino acid extracellular domain, a 27 amino acid transmembrane domain and a 42 amino acid cytoplasmic domain. Recombinant human soluble 4-1BB Receptor is a 167 amino acid polypeptide (17.7 kDa), which contains the cysteine rich TNFR-like extracellular domain of 4-1BB Receptor. |
, Adult Mouse) Bone Dissociation System 4 (Osteoblasts), Adult Mouse |
|
4-20214 |
CHI Scientific |
ea |
Ask for price |
, Rat) Liver Dissociation System 4 (Hepatocytes, rat), Rat |
|
4-20304 |
CHI Scientific |
ea |
Ask for price |
, Adult mouse) Pituitary Dissociation System 4 (Pituitary), Adult mouse |
|
4-20404 |
CHI Scientific |
ea |
Ask for price |
, Adult rat) Thymus Dissociation System 4 (Epithelial), Adult rat |
|
4-20444 |
CHI Scientific |
ea |
Ask for price |
, Mouse and Rat) Cartilage Dissociation System 4 (Chondrocytes), Mouse and Rat |
|
4-20234 |
CHI Scientific |
ea |
Ask for price |
, Mouse) Mammary Dissociation System 4 (Epithelial, Swiss 3T3), Mouse |
|
4-20324 |
CHI Scientific |
ea |
Ask for price |
, Mouse and Rat) Pancreas Dissociation System 4 (Islets), Mouse and Rat |
|
4-20374 |
CHI Scientific |
ea |
Ask for price |
, Mouse and Rat) Skin Dissociation System 4 (Keratinocytes), Mouse and Rat |
|
4-20434 |
CHI Scientific |
ea |
Ask for price |
 DIGITAL SHAKER FOR 4 MICROPLATES, 230V-EU PLUG |
|
6781-4 |
CORNING |
1/pk |
EUR 798 |
|
Description: Lab Equipment; Shakers & Mixers |
 DIGITAL SHAKER FOR 4 MICROPLATES, 230V-UK PLUG |
|
6782-4 |
CORNING |
1/pk |
EUR 798 |
|
Description: Lab Equipment; Shakers & Mixers |
, Mouse and Rat) Adipose/Fat Dissociation System 4 (Predipocytes), Mouse and Rat |
|
4-20194 |
CHI Scientific |
ea |
Ask for price |
, Mouse and Rat) Brain Dissociation System 4 (Endothelial, Microvessels), Mouse and Rat |
|
4-20224 |
CHI Scientific |
ea |
Ask for price |
, Mouse and Rat) Endothelial Dissociation System 4 (Endothelial,Cerebral), Mouse and Rat |
|
4-20244 |
CHI Scientific |
ea |
Ask for price |
, Mouse and Rat) Heart Dissociation System 4 (Myocytes,Ventricles), Mouse and Rat |
|
4-20274 |
CHI Scientific |
ea |
Ask for price |
, Mouse and Rat) Muscle Dissociation System 4 (Smooth muscle), Mouse and Rat |
|
4-20334 |
CHI Scientific |
ea |
Ask for price |
, Mouse and Rat) Parotid Dissociation System 4 (Parotid acinar), Mouse and Rat |
|
4-20394 |
CHI Scientific |
ea |
Ask for price |
, Mouse and Rat) Epithelial Dissociation System 4 (Epithelial,Submandibular salivary), Mouse and Rat |
|
4-20254 |
CHI Scientific |
ea |
Ask for price |
, Mouse and Rat) Heart Dissociation System 4 (Retinal pigment epithelial), Mouse and Rat |
|
4-20294 |
CHI Scientific |
ea |
Ask for price |
, Mouse and Rat) Lung Dissociation System 4 (Alveolar type II), Mouse and Rat |
|
4-20314 |
CHI Scientific |
ea |
Ask for price |
, Mouse and Rat) Reproductive Tissue Dissociation System 4 (Epithelial, vagina), Mouse and Rat |
|
4-20414 |
CHI Scientific |
ea |
Ask for price |
) ExoStd? Lyophilized Exosome Standard (30 µg, Human Plasma, 4 vials) |
|
M1040-4 |
Biovision |
|
EUR 909 |
) ExoStd? Lyophilized Exosome Standard (100 µg, Human Plasma, 4 vials) |
|
M1041-4 |
Biovision |
|
EUR 1137 |
) ExoStd? Lyophilized Exosome Standard (30 µg, Human Serum, 4 vials) |
|
M1042-4 |
Biovision |
|
EUR 909 |
) ExoStd? Lyophilized Exosome Standard (100 µg, Human Serum, 4 vials) |
|
M1043-4 |
Biovision |
|
EUR 1132 |
) ExoStd? Lyophilized Exosome Standard (30 µg, Human Urine, 4 vials) |
|
M1044-4 |
Biovision |
|
EUR 909 |
) ExoStd? Lyophilized Exosome Standard (100 µg, Human Urine, 4 vials) |
|
M1045-4 |
Biovision |
|
EUR 1137 |
) ExoStd? Lyophilized Exosome Standard (30 µg, Human Saliva, 4 vials) |
|
M1046-4 |
Biovision |
|
EUR 914 |
) ExoStd? Lyophilized Exosome Standard (100 µg, Human Saliva, 4 vials) |
|
M1047-4 |
Biovision |
|
EUR 1137 |
) ExoStd? Lyophilized Exosome Standard (30 µg, U87 MG, 4 vials) |
|
M1054-4 |
Biovision |
|
EUR 914 |
) ExoStd? Lyophilized Exosome Standard (100 µg, U87 MG, 4 vials) |
|
M1055-4 |
Biovision |
|
EUR 1142 |
, Mouse and Rat) Adrenal Dissociation System 4 (Heart, Adrenal chromaffin, Paraneurons), Mouse and Rat |
|
4-20204 |
CHI Scientific |
ea |
Ask for price |
, Mouse and Rat) Kidney Dissociation System 4 (Inner medullary duct,Papillae), Mouse and Rat |
|
4-29294 |
CHI Scientific |
ea |
Ask for price |
) ExoStd? Lyophilized Exosome Standard (30 µg, COLO1 cell line, 4 vials) |
|
M1048-4 |
Biovision |
|
EUR 914 |
) ExoStd? Lyophilized Exosome Standard (100 µg, COLO1 cell line, 4 vials) |
|
M1049-4 |
Biovision |
|
EUR 1142 |
) ExoStd? Lyophilized Exosome Standard (30 µg, MM1 cell line, 4 vials) |
|
M1050-4 |
Biovision |
|
EUR 914 |
) ExoStd? Lyophilized Exosome Standard (100 µg, MM1 cell line, 4 vials) |
|
M1051-4 |
Biovision |
|
EUR 1142 |
) ExoStd? Lyophilized Exosome Standard (30 µg, BLCL21 cell line, 4 vials) |
|
M1052-4 |
Biovision |
|
EUR 914 |
) ExoStd? Lyophilized Exosome Standard (100 µg, BLCL21 cell line, 4 vials) |
|
M1053-4 |
Biovision |
|
EUR 1142 |
) ExoStd? Lyophilized Exosome Standard (30 µg, HCT116 cell line, 4 vials) |
|
M1058-4 |
Biovision |
|
EUR 914 |
) ExoStd? Lyophilized Exosome Standard (100 µg, HCT116 cell line, 4 vials) |
|
M1059-4 |
Biovision |
|
EUR 1142 |
) ExoStd? Lyophilized Exosome Standard (30 µg, PC3 cell line, 4 vials) |
|
M1060-4 |
Biovision |
|
EUR 914 |
) ExoStd? Lyophilized Exosome Standard (100 µg, PC3 cell line, 4 vials) |
|
M1061-4 |
Biovision |
|
EUR 1142 |
) ExoStd? Lyophilized Exosome Standard (30 µg, DAUD1 cell line, 4 vials) |
|
M1064-4 |
Biovision |
|
EUR 914 |
) ExoStd? Lyophilized Exosome Standard (100 µg, DAUD1 cell line, 4 vials) |
|
M1065-4 |
Biovision |
|
EUR 1142 |
) ExoStd? Lyophilized Exosome Standard (30 µg, A549 cell line, 4 vials) |
|
M1066-4 |
Biovision |
|
EUR 914 |
) ExoStd? Lyophilized Exosome Standard (100 µg, A549 cell line, 4 vials) |
|
M1067-4 |
Biovision |
|
EUR 1142 |
) ExoStd? Lyophilized Exosome Standard (30 µg, B16F10 cell line, 4 vials) |
|
M1070-4 |
Biovision |
|
EUR 914 |
) ExoStd? Lyophilized Exosome Standard (100 µg, B16F10 cell line, 4 vials) |
|
M1071-4 |
Biovision |
|
EUR 1142 |
, Mouse and Rat) Tumor Dissociation System 4 (Epithelial tumor,Neoplastic, Tumor, islet), Mouse and Rat |
|
4-20484 |
CHI Scientific |
ea |
Ask for price |
) ExoStd? Lyophilized Exosome Standard (30 µg, BPH-1 cell line, 4 vials) |
|
M1062-4 |
Biovision |
|
EUR 914 |
) ExoStd? Lyophilized Exosome Standard (100 µg, BPH-1 cell line, 4 vials) |
|
M1063-4 |
Biovision |
|
EUR 1142 |
) ExoStd? Lyophilized Exosome Standard (30 µg, K-562 cell line, 4 vials) |
|
M1068-4 |
Biovision |
|
EUR 914 |
) ExoStd? Lyophilized Exosome Standard (100 µg, K-562 cell line, 4 vials) |
|
M1069-4 |
Biovision |
|
EUR 1142 |
) ExoStd? Lyophilized Exosome Standard (30 µg, SK-N-SH cell line, 4 vials) |
|
M1056-4 |
Biovision |
|
EUR 914 |
) ExoStd? Lyophilized Exosome Standard (100 µg, SK-N-SH cell line, 4 vials) |
|
M1057-4 |
Biovision |
|
EUR 1142 |
, Mouse and Rat) Neural Dissociation System 4 (Cerebella neurons Dorsa horn neurons Neurons, sympathetic Precursor Spinal), Mouse and Rat |
|
4-20354 |
CHI Scientific |
ea |
Ask for price |
 ELISA Kit) Human Carbohydrate Antigen 72-4 (CA72-4) ELISA Kit |
|
DLR-CA72-4-Hu-48T |
DL Develop |
48T |
EUR 479 |
|
|
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Carbohydrate Antigen 72-4 (CA72-4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Human Carbohydrate Antigen 72-4 (CA72-4) ELISA Kit |
|
DLR-CA72-4-Hu-96T |
DL Develop |
96T |
EUR 621 |
|
|
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Carbohydrate Antigen 72-4 (CA72-4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Human Carbohydrate Antigen 72-4 (CA72-4) ELISA Kit |
|
RD-CA72-4-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 478 |
Human Carbohydrate Antigen 72-4 (CA72-4) ELISA Kit |
|
RD-CA72-4-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 662 |
Human Carbohydrate Antigen 72-4 (CA72-4) ELISA Kit |
|
RDR-CA72-4-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 500 |
Human Carbohydrate Antigen 72-4 (CA72-4) ELISA Kit |
|
RDR-CA72-4-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 692 |
 ELISA test) PRL (Prolactin) ELISA test |
|
4 |
Biobase |
96T/Box |
Ask for price |
|
|
|
Description: ELISA based test for quantitative detection of PRL (Prolactin) |
) Anti-p53 Monoclonal Antibody (PAb122) |
|
M00001-4 |
BosterBio |
200ug |
EUR 511 |
|
Description: Mouse Monoclonal p53 Antibody (PAb122). Validated in IP, IF, IHC, WB and tested in Human, Mouse, Rat. |
 Rabbit Monoclonal Antibody, Clone#RM316) Anti-AKT (PH domain) Rabbit Monoclonal Antibody, Clone#RM316 |
|
M00024-4 |
BosterBio |
100uL |
EUR 385 |
|
Description: Anti-AKT (PH domain) Rabbit Monoclonal Antibody, Clone#RM316 tested in WB, IHC, reactive to Human |
 Anti-CDK1/Cdc2 Rabbit Monoclonal Antibody |
|
M00209-4 |
BosterBio |
100ug/vial |
EUR 397 |
|
Description: Anti-CDK1 Rabbit Monoclonal Antibodytested for IP, IF, IHC, ICC, WB in Human, Mouse, Rat |
 Anti-GAPDH Antibody |
|
M00227-4 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal GAPDH Antibody. Validated in IF, IHC, WB and tested in Bovine, Chicken, Equine, Human, Mouse, Pig, Rat. |
 Anti-Vimentin Antibody |
|
M00235-4 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Vimentin Antibody. Validated in IHC, WB and tested in Bovine, Equine, Human, Mouse, Pig, Rat. |
 Anti-Aurora A Kinase Monoclonal Antibody |
|
M00246-4 |
BosterBio |
100ul |
EUR 397 |
|
Description: Mouse Monoclonal Aurora A Kinase Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat. |
 Anti-Ki-67 Antibody |
|
M00254-4 |
BosterBio |
100ul |
EUR 397 |
|
Description: Chicken Polyclonal Ki-67 Antibody. Validated in WB and tested in Human, Mouse, Rat. |
 Anti-C-FOS Antibody |
|
M00297-4 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal C-FOS Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat. |
 Anti-active + pro Caspase 3 Rabbit Monoclonal Antibody |
|
M00334-4 |
BosterBio |
100ug/vial |
EUR 397 |
|
Description: Rabbit Monoclonal active + pro Caspase 3 Antibody. Validated in IP, IHC, WB and tested in Human, Mouse, Rat. |
 Anti-Thyroglobulin Monoclonal Antibody |
|
M00359-4 |
BosterBio |
100ug |
EUR 397 |
|
Description: Rabbit Monoclonal Thyroglobulin Antibody. Validated in IP, IF, WB and tested in Human. |
 Rabbit Monoclonal Antibody, Clone#RM341) Anti-CK-5/CK-6 (Cytokeratin 5/ Cytokeratin 6) Rabbit Monoclonal Antibody, Clone#RM341 |
|
M00398-4 |
BosterBio |
100uL |
EUR 385 |
|
Description: Anti-CK-5/CK-6 (Cytokeratin 5/ Cytokeratin 6) Rabbit Monoclonal Antibody, Clone#RM341 tested in WB, IHC, reactive to Human |
 Rabbit Monoclonal Antibody, Clone#RM254) Anti-AR (N-terminal) Rabbit Monoclonal Antibody, Clone#RM254 |
|
M00542-4 |
BosterBio |
100uL |
EUR 427 |
|
Description: Anti-AR (N-terminal) Rabbit Monoclonal Antibody, Clone#RM254 tested in WB, IHC, reactive to Human |
 Anti-HSP27 Monoclonal Antibody |
|
M00676-4 |
BosterBio |
200ug |
EUR 397 |
|
Description: Mouse Monoclonal HSP27 Antibody. Validated in ELISA, IP, IF, WB and tested in Human. |
 Anti-Cyclin B1 Rabbit Monoclonal Antibody, Clone#RM281 |
|
M00745-4 |
BosterBio |
100uL |
EUR 375 |
|
Description: Anti-Cyclin B1 Rabbit Monoclonal Antibody, Clone#RM281 tested in WB, IHC, reactive to Human |
) Anti-ZAP70 Antibody (monoclonal, 6F12) |
|
M00754-4 |
BosterBio |
100ug/vial |
EUR 294 |
 Rabbit Monoclonal Antibody, Clone#RM278) Anti-Aurora-B (AURKB) Rabbit Monoclonal Antibody, Clone#RM278 |
|
M00762-4 |
BosterBio |
100uL |
EUR 375 |
|
Description: Anti-Aurora-B (AURKB) Rabbit Monoclonal Antibody, Clone#RM278 tested in WB, IHC, reactive to Human |
 Anti-ACTA2 Rabbit Monoclonal Antibody, Clone#RM253 |
|
M01072-4 |
BosterBio |
100ul |
EUR 375 |
|
Description: Anti-ACTA2 Rabbit Monoclonal Antibody, Clone#RM253 tested in WB, IHC, reactive to Human, Mouse, (Bovine, Rat) |
) Anti-Cytokeratin 18 Antibody (monoclonal, 7I6) |
|
M01357-4 |
BosterBio |
100ug/vial |
EUR 334 |
 Rabbit Monoclonal Antibody, Clone#RM266) Anti-CK8 (Cytokeratin-8) Rabbit Monoclonal Antibody, Clone#RM266 |
|
M01421-4 |
BosterBio |
100uL |
EUR 375 |
|
Description: Anti-CK8 (Cytokeratin-8) Rabbit Monoclonal Antibody, Clone#RM266 tested in WB, IHC, reactive to Human |
 Anti-GAP43 Antibody |
|
M01868-4 |
BosterBio |
100ul |
EUR 397 |
|
Description: Chicken Polyclonal GAP43 Antibody. Validated in IF, IHC, WB and tested in Bovine, Chicken, Equine, Human, Mouse, Pig, Rat. |
 Rabbit Monoclonal Antibody) Anti-Actin (Alpha-Actin) Rabbit Monoclonal Antibody |
|
M02014-4 |
BosterBio |
100ug/vial |
EUR 397 |
|
Description: Rabbit Monoclonal Actin (Alpha-Actin) Antibody. Validated in Flow Cytometry, IP, IF, IHC, ICC, WB and tested in Human, Mouse, Rat. |
 Rabbit Monoclonal Antibody, Clone#RM364) Anti-Cytokeratin 19 (CK-19) Rabbit Monoclonal Antibody, Clone#RM364 |
|
M02101-4 |
BosterBio |
100uL |
EUR 385 |
|
Description: Anti-Cytokeratin 19 (CK-19) Rabbit Monoclonal Antibody, Clone#RM364 tested in WB, IHC, reactive to Human |
 Anti-Neurofilament Light Chain NF-L Antibody |
|
M02482-4 |
BosterBio |
100ul |
EUR 397 |
|
Description: Chicken Polyclonal Neurofilament Light Chain NF-L Antibody. Validated in IHC, WB and tested in Bovine, Human, Mouse, Pig, Rat. |
 Anti-Ubiquitin Monoclonal Antibody |
|
M02848-4 |
BosterBio |
100ul |
EUR 397 |
|
Description: Mouse Monoclonal Ubiquitin Antibody. Validated in IHC, WB and tested in Bovine, C. Elegans, Chicken, Drosophila, Equine, Mouse, Pig. |
The higher cost per sample on the MinION platform ($71.56) versus the MiSeq platform ($67.83) was attributed to differences in batching capabilities.Our study demonstrates the interchangeability of MiSeq and MinION platforms for generation of accurate and actionable results for the treatment of tuberculosis.
