Business myeloid next-generation sequencing (NGS) panels might facilitate uniform era of uncooked information between laboratories. Nevertheless, completely different methods for information filtering and variant annotation might contribute to variations in variant detection and reporting. Right here, we current how customized information filtering or the usage of Oncomine prolonged information filtering enhance detection of clinically related mutations with the Oncomine Myeloid Analysis Assay. The research included all affected person samples (n = 264) analyzed throughout the first-year, single-site, scientific use of the Ion Torrent Oncomine Myeloid Analysis Assay.
In information evaluation, the default evaluation filter was supplemented with our personal information filtering algorithm with a purpose to detect further clinically related mutations. As well as, we developed a delicate supplementary take a look at for the ASXL1 c.1934dupG p.Gly646fs mutation by fragment evaluation. Utilizing our customized filter chain, we discovered 96 completely different reportable variants that weren’t detected by the default filter chain. Twenty-six of those have been labeled as variants of sturdy or potential scientific significance (tier I/tier II variants), and the customized filtering found in any other case undetected tier I/tier II variants in 25 of 132 sufferers with clinically related mutations (19%).
The remaining 70 variants not detected by the default filter chain have been labeled as variants of unknown significance. Amongst these have been a number of distinctive variants with attainable pathogenic potential judged by bioinformatic predictions. The lately launched Oncomine 5.14 prolonged filter algorithm detects most however not the entire tier I/tier II variants that weren’t detected by the default filter. The supplementary fragment evaluation for the ASXL1 c.1934dupG p.Gly646fs confidently detected a variant allele frequency of all the way down to 4.8% (SD 0.83%). The assay additionally detected the ASXL1 c.1900_1922del23 mutation.
Detection of clinically related variants with the Oncomine Myeloid Analysis NGS assay will be considerably improved by supplementing the default filter chain with customized information filtering or the lately launched Oncomine 5.14 prolonged filter algorithm. Our accent fragment evaluation facilitates straightforward testing for frequent ASXL1 mutations which might be poorly or not coated by the NGS assay.
Lowered PCR-generated errors from a hybrid capture-based NGS assay for HLA typing
Subsequent era sequencing (NGS) assays are state-of-the-art for HLA genotyping. To sequence on an Illumina sequencer, the DNA of curiosity should be enriched, fragmented, and bookended with identified oligonucleotide sequences, a course of referred to as library building. Many HLA genotyping assays enrich the goal loci by long-range PCR (LR-PCR), previous to fragmentation. This PCR step has been reported to introduce errors within the DNA to be sequenced, together with inaccurate replication of repeated sequences, and the in vitro recombination of alleles encoded on separate chromosomes.
Another library building technique includes fragmentation of genomic DNA, adopted by hybrid-capture (HC) enrichment of goal HLA loci. This HC-based technique includes PCR, however with far fewer cycles. Consequently, the HC technique had considerably fewer PCR-induced errors, together with extra devoted replication of repeated sequences, and the close to elimination of recombinant sequences. These enhancements possible produce extra correct NGS sequencing information of HLA loci. The Ng-H and Ng’-H contacts vary, actually, between sturdy covalent bonds to weak, non-covalent interactions, and their common variability clearly illustrates the peculiar functionality of the noble gases to bear interactions masking your entire spectrum of the chemical bond.
The construction, stability, and bonding character of fifteen (Ng-H-Ng)+ and (Ng-H-Ng’)+ (Ng, Ng’ = He-Xe) compounds have been explored by theoretical calculations carried out on the coupled cluster stage of idea. The character of the stabilizing interactions was, specifically, assayed utilizing a technique lately proposed by the authors to categorise the chemical bonds involving the noble-gas atoms. The bond distances and dissociation energies of the investigated ions fall in relatively massive intervals, and observe common periodic tendencies, clearly referable to the distinction between the proton affinity (PA) of the varied Ng and Ng’. These variations are properly correlated with the bonding state of affairs of the (Ng-H-Ng)+ and (Ng-H-Ng’)+.
NGS-Based mostly Utility for Routine Non-Invasive Pre-Implantation Genetic Evaluation in IVF
Though non-invasive pre-implantation genetic testing for aneuploidy (NIPGT-A) is probably applicable to evaluate chromosomal ploidy of the embryo, sensible software of it in a routine IVF centre haven’t been began within the absence of a suggestion. Our goal on this research was to offer a complete workflow for a clinically relevant technique for NIPGT-A based mostly on next-generation sequencing (NGS) expertise with the corresponding bioinformatic pipeline. In a retrospective research, we carried out NGS on spent blastocyst tradition media of Day three embryos fertilised with intracytoplasmic sperm injection (ICSI) with high quality rating on morphology evaluation utilizing the clean tradition media as background management.
UV Transilluminator |
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| 50060101 | Vision Med Tech | 1 Unit | Ask for price |
UV Transilluminator |
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| 50060102 | Vision Med Tech | 1 Unit | Ask for price |
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UV LED Transilluminator |
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UltraBright UV Transilluminator |
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| MB-16 | MaestroGen | each | EUR 410 |
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Description: 302 nm, adjustable, 160 x 200 viewing |
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UltraBright UV Transilluminator |
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| MB-21 | MaestroGen | each | EUR 460 |
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Description: 302 nm, adjustable, 210 x 260 viewing |
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UltraBright UV Transilluminator |
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| MLB-16 | MaestroGen | each | EUR 450 |
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Description: 302/365 nm, adjustable, 160 x 200 viewing |
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UltraBright UV Transilluminator |
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| MLB-21 | MaestroGen | each | EUR 520 |
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Description: 302/365 nm, adjustable, 210 x 260 viewing |
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UV transilluminator BGEL-104 |
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| BGEL-104 | Biolab Scientific Ltd. | Each | Ask for price |
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Description: Gel instrument is a technique used in laboratory to separate charged molecules like DNA, RNA and proteins according to their size. Gel electrophoresis instruments are commonly used to run and compare DNA samples. - Humanized design of the whole machine, the UV protection board can be stepless adjust at any angle and positioning, to ensure the best protection of UV light and does not affect the observation. - Compact sealing structure design ensures that the cutting operation and the cleaning and cutting platform are free of leakage, which greatly reduces the possibility of gel contamination and internal damage caused by leakage and greatly improves the service life of the UV cutting instrument. - The special UV filter glass has good permeability to specific wavelength UV, which can ensure higher detection sensitivity and enhance the signal capture capability of the weak band. - Reasonable structural design and high quality UV lamp. |
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UV LED Transilluminator (200*130mm 2) |
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UV Transilluminator 302nm 15x11 - EACH |
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UltraSlim™ UV Transilluminator |
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| UUV-01 | MaestroGen | each | EUR 295 |
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Description: 302 nm, for mini gels, 150X 80 viewing |
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UltraBright UV Transilluminator, 302 nm |
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| QMB-16 | ABM | 1 | EUR 1050 |
UltraBright UV Transilluminator, 302/365 nm |
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| Q-MLB-16 | ABM | 1 | EUR 1050 |
WUV-M20, UV Transilluminator, 312nm, 100V |
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| 3532197 | Atto | 1unit | EUR 1149.72 |
WUV-M20, UV Transilluminator, 312nm, 220V |
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| 3532198 | Atto | 1unit | EUR 1149.72 |
UV Transilluminator 20 x 20 cm 302nm - EACH |
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| ELE7584 | Scientific Laboratory Supplies | EACH | EUR 1274.4 |
UV Transilluminator 25 x 30 cm 302nm - EACH |
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| ELE7586 | Scientific Laboratory Supplies | EACH | EUR 1742.85 |
MyView™ Compact UV Transilluminator, 115 V |
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| BM0298 | GenDepot | Ea | EUR 1100 |
MyView™ Compact UV Transilluminator, 230 V |
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| BM0299 | GenDepot | Ea | EUR 1100 |
UV Transilluminator 21 x 21cm 254nm - EACH |
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| ULT4500 | Scientific Laboratory Supplies | EACH | EUR 1455.3 |
UV Transilluminator 21 x 21cm 312nm - EACH |
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| ULT4502 | Scientific Laboratory Supplies | EACH | EUR 1386.45 |
UV Transilluminator 21 x 21cm 365nm - EACH |
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| ULT4504 | Scientific Laboratory Supplies | EACH | EUR 1386.45 |
UV Transilluminator 21 x 26cm 254nm - EACH |
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UV Transilluminator 21 x 26cm 312nm - EACH |
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UV Transilluminator 21 x 26cm 365nm - EACH |
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| ULT4514 | Scientific Laboratory Supplies | EACH | EUR 1899.16 |
UltraBright UV Transilluminator, 302 nm (Large View) |
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| QMB-21 | ABM | 1 | EUR 1150 |
UltraBright UV Transilluminator, 302/365 nm (Large View) |
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| Q-MLB-21 | ABM | 1 | EUR 1150 |
uv transilluminator 20x20cm, 312nm, 50/100% switch |
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| UVTR2200 | Consort | ea | EUR 1393.2 |
Mini-Imager Dual UV/LED Transilluminator, 302 nm/470 nm |
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| Q-MI-01 | ABM | 1 | EUR 4650 |
Transilluminator 365nm - EACH |
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| ELE0032 | Scientific Laboratory Supplies | EACH | EUR 1205.88 |
Transilluminator 302nm - EACH |
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| ELE0034 | Scientific Laboratory Supplies | EACH | EUR 1043.55 |
Transilluminator 254nm - EACH |
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Transilluminator 302nm - EACH |
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UV Transilluminator 21 x 21cm Dual Wave 254/365nm - EACH |
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UV Transilluminator 21 x 21cm Dual Wave 254/312nm - EACH |
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| ULT4508 | Scientific Laboratory Supplies | EACH | EUR 1452.6 |
UV Transilluminator 21 x 26cm Dual Wave 254/365nm - EACH |
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| ULT4516 | Scientific Laboratory Supplies | EACH | EUR 1971.13 |
Blue LED Transilluminator |
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BLOOK, LED Transilluminator |
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SmartBlue™ Transilluminator |
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| E4000 | Benchmark Scientific | 1 each | EUR 665.24 |
Mini Blue LED Transilluminator |
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pBLOOK, LED Transilluminator, Red |
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UltraBright LED Transilluminator |
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| LB-16 | MaestroGen | each | EUR 420 |
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Description: 470nm Blue LED, 160 x 200 viewing |
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UV transilluminators |
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| E3333 | Beyotime Biotech Inc | 1 Unit | Ask for price |
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UV transilluminators |
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EZ-Viewer LED Transilluminator |
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| LED001 | Shanghai WSHT Biotechnology | set | EUR 624 |
Blue Light Transilluminator - EACH |
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| ELE3416 | Scientific Laboratory Supplies | EACH | EUR 868.05 |
pBLOOK, LED Transilluminator, Pink |
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| BK002-00PK | GeneDireX | 1 Set | Ask for price |
UltraBright LED Transilluminator, 470 nm |
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| QLB-16 | ABM | 1 | EUR 1050 |
pBLOOK, LED Transilluminator, Black |
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pBLOOK, LED Transilluminator, White |
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μBLOOK, LED Transilluminator, White |
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SmartBlue™ Transilluminator, 230V input |
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| E4000-E | Benchmark Scientific | 1 PC | EUR 665.24 |
microDOC System with UV Transilluminator UVTS365 21x21cm 365nm - EACH |
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| MOL3506 | Scientific Laboratory Supplies | EACH | EUR 6189.75 |
SmartBlue™ Mini Transilluminator |
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| E4100 | Benchmark Scientific | 1 each | EUR 491.64 |
DUALED Blue/White Transilluminator |
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pBLOOK, LED Transilluminator, Purple |
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pBLOOK, LED Transilluminator, Yellow |
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TRANSILLUMINATOR LED 20X12 CM + HOOD |
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| BLOOK | Consort | ea | EUR 780 |
Transilluminator 20x20cm 302/365 - EACH |
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| ELE2104 | Scientific Laboratory Supplies | EACH | EUR 1421.55 |
Transilluminator 25x30cm 302/365 - EACH |
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| ELE2106 | Scientific Laboratory Supplies | EACH | EUR 2018.25 |
pBLOOK, LED Transilluminator, Navy Blue |
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| BK002-00BU | GeneDireX | 1 Set | Ask for price |
SmartBlue™ Mini Transilluminator, 230V input |
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| E4100-E | Benchmark Scientific | 1 each | EUR 491.64 |
SmartDoc band pass filter, 590nm, for imaging EtBr on UV transilluminator |
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| E5001-590 | Benchmark Scientific | 1 PC | EUR 137.39 |
BluePad - Blue/White LED Transilluminator |
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| Bp001CU | GeneOn | 1 pcs | EUR 779 |
pBLOOK, LED Transilluminator, Tiffany Blue |
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| BK002-00BG | GeneDireX | 1 Set | Ask for price |
Transilluminator Tube for ULT40020622 - EACH |
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| ULT4042 | Scientific Laboratory Supplies | EACH | EUR 37.8 |
Bandpeeper LED Transilluminator BGEL-202 |
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| BGEL-202 | Biolab Scientific Ltd. | Each | Ask for price |
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Description: Gel instrument is a technique used in laboratory to separate charged molecules like DNA, RNA and proteins according to their size. Gel electrophoresis instruments are commonly used to run and compare DNA samples. - Convenience-Saving time and cost for new sample electrophoresis by trial & error - Accuracy-Accurate electrophoresis duration control - Sensitivity-2ng nucleic acid - Powerfulness-Powerful blue LEDs matched with common nucleic acid dyes |
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UltraBright Blue/Green LED Transilluminator |
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| LB-18 | MaestroGen | each | EUR 550 |
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Description: 505nm Blue/Green LED, 160 x 200 viewing |
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Ultra Bright Blue LED Transilluminator - EACH |
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| ELE7526 | Scientific Laboratory Supplies | EACH | EUR 1252.8 |
SmartDoc band pass filter, 535nm, for imaging Green Stains on UV transilluminator |
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| E5001-535 | Benchmark Scientific | 1 PC | EUR 137.39 |
Ultra bright LED transilluminator BGEL-204 |
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| BGEL-204 | Biolab Scientific Ltd. | Each | Ask for price |
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Description: Gel instrument is a technique used in laboratory to separate charged molecules like DNA, RNA and proteins according to their size. Gel electrophoresis instruments are commonly used to run and compare DNA samples. - Wavelengths-Three wavelengths: Blue 470nm, Green 530nm and Red 630nm - Accuracy-CV<5% across the viewing area - Sensitivity-High contrast, up to 1 ng nucleic acid - Safety-No damage to you and your sample |
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SmartDoc band UV Blocking Filter, optimized for use with E3100 transilluminator |
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| E5001-UVBLOCK | Benchmark Scientific | 1 each | EUR 55.59 |
UVPAD, LED Transilluminator, with mini Darkroom |
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| UP001CU | Bio-Helix | Set | EUR 900 |
Orange filter cover for mini LED transilluminator |
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| E1200-OC | Benchmark Scientific | 1 each | EUR 40.1 |
BluPAD Dual LED Blue/White Light Transilluminator |
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| MBS402003-1Set | MyBiosource | 1Set | EUR 575 |
Dual LED Transilluminator Gel Documentation System |
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| abx795001-10nmol | Abbexa | 10 nmol | Ask for price |
Dual LED Transilluminator Gel Documentation System |
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| abx795001-5nmol | Abbexa | 5 nmol | EUR 962.5 |
Ultra bright blue/green LED transilluminator BGEL-201 |
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| BGEL-201 | Biolab Scientific Ltd. | Each | Ask for price |
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Description: Gel instrument is a technique used in laboratory to separate charged molecules like DNA, RNA and proteins according to their size. Gel electrophoresis instruments are commonly used to run and compare DNA samples. - Accuracy-CV<5% across the viewing area - Sensitivity-High contrast, up to 1 ng nucleic acid - Safety-No damage to you and your sample - Ease-Easy for observation and gel cutting |
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Accuris SmartDoc UV Blocking Mat - EACH |
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| ELE1252 | Scientific Laboratory Supplies | EACH | EUR 70.2 |
Accuris |
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| W1105-20 | Benchmark Scientific | 1 PC | EUR 91.92 |
Accuris |
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| W1105-500 | Benchmark Scientific | 1 PC | EUR 118.9 |
Accuris |
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| W1105-9-1000 | Benchmark Scientific | 1 PC | EUR 223.3 |
Accuris myGel Mini Electrophoresis Syst w/ Transf - EACH |
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| ELE1111 | Scientific Laboratory Supplies | EACH | EUR 961.2 |
Accuris Taq Plus, 250 units |
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| PR1000-TP-250 | Benchmark Scientific | 1 PC | EUR 201.3 |
Accuris Taq Plus, 500 units |
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| PR1000-TP-500 | Benchmark Scientific | 1 PC | EUR 322.5 |
Accuris NextPette Carousel - EACH |
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| PIP1348 | Scientific Laboratory Supplies | EACH | EUR 276.13 |
Accuris Hydrophobic Filter - EACH |
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| ASP1092 | Scientific Laboratory Supplies | EACH | EUR 48.87 |
Accuris 5mL Pipette Tips - PK100 |
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| PIP1350 | Scientific Laboratory Supplies | PK100 | EUR 79.65 |
Accuris Taq Polymerase, 1000 units |
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| PR1000-1000 | Benchmark Scientific | 1 PC | EUR 172.11 |
Accuris Taq Polymerase, 500 units |
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| PR1000-500 | Benchmark Scientific | 1 PC | EUR 106.71 |
Accuris Taq Polymerase, 6000 units |
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| PR1000-6000 | Benchmark Scientific | 1 PC | EUR 878.9 |
Accuris qMax Probe,100 reactions |
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| PR2001-N-100 | Benchmark Scientific | 1 PC | EUR 80.03 |
Accuris 10mL Pipette Tips - PK100 |
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| PIP1349 | Scientific Laboratory Supplies | PK100 | EUR 167.4 |
Accuris Orange Viewing Cover - EACH |
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| ELE1256 | Scientific Laboratory Supplies | EACH | EUR 47.25 |
Accuris Hot Start Taq, 1000 units |
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| PR1000-HS-1000 | Benchmark Scientific | 1 PC | EUR 383.06 |
Accuris Hot Start Taq, 500 units |
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| PR1000-HS-500 | Benchmark Scientific | 1 PC | EUR 221.86 |
Accuris Hot Start Taq, 6000 units |
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| PR1000-HS-6000 | Benchmark Scientific | 1 PC | EUR 1848.75 |
Accuris Taq Plus, sample, 50 units |
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| PR1000-TP-S | Benchmark Scientific | 1 PC | EUR 7.1 |
Accuris Extra Tubing Set 1m - EACH |
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| ASP1096 | Scientific Laboratory Supplies | EACH | EUR 47.25 |
Filter for Accuris Microplate Reader |
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| MR9600-490 | Benchmark Scientific | 1 PC | EUR 241.56 |
Filter for Accuris Microplate Reader |
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| MR9600-646 | Benchmark Scientific | 1 PC | EUR 241.56 |
Accuris SmartDoc 2.0 Imaging Enclosure |
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| E5001-SD | Benchmark Scientific | 1 PC | EUR 466.65 |
Accuris SmartBlue Viewing Glasses - EACH |
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Accuris Taq Polymerase, sample, 50 units |
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| PR1000-S | Benchmark Scientific | 1 PC | EUR 7.31 |
Accuris A Balance 210g Read 0.0001g - EACH |
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Accuris Hot Start Taq, sample, 50 units |
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| PR1000-HS-S | Benchmark Scientific | 1 PC | EUR 7.31 |
Accuris M1 Weight Set 1g-1000g - EACH |
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| BAL1886 | Scientific Laboratory Supplies | EACH | EUR 429.3 |
Accuris Option Quick Disconnect Kit - EACH |
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| ASP1093 | Scientific Laboratory Supplies | EACH | EUR 216 |
Accuris QuickSilver TBE Buffer Powder - PK50 |
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Accuris SmartDoc 2.0 Imaging Enclosure - EACH |
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| ELE1250 | Scientific Laboratory Supplies | EACH | EUR 673.65 |
Accuris High Fidelity Master Mix, 200 rxns |
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| PR1001-HF-200 | Benchmark Scientific | 1 each | EUR 180.66 |
Accuris High Fidelity Master Mix, 500 rxns |
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| PR1001-HF-500 | Benchmark Scientific | 1 each | EUR 401.29 |
Accuris Reversible Combs 22/12 Teeth - PK2 |
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Accuris Reversible Combs 18/10 Teeth - PK2 |
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Accuris QuickSilver TAE Buffer Powder - PK100 |
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| EB1000 | Scientific Laboratory Supplies | PK100 | EUR 352.35 |
Accuris QuickSilver PBS Buffer Powder - PK100 |
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| EB1200 | Scientific Laboratory Supplies | PK100 | EUR 224.1 |
Accuris QuickSilver TBS Buffer Powder - PK100 |
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Accuris Graduated PC Collection Bottle - EACH |
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| ASP1094 | Scientific Laboratory Supplies | EACH | EUR 236.25 |
Accuris myVolt Mini Power Supply 230V - EACH |
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| ELE1534 | Scientific Laboratory Supplies | EACH | EUR 361.8 |
Accuris™ M1 Grade Calibration Weight, 1g |
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| W1005-1 | Benchmark Scientific | 1 each | EUR 12.88 |
Accuris™ M1 Grade Calibration Weight, 2g |
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| W1005-2 | Benchmark Scientific | 1 each | EUR 12.88 |
Accuris™ M1 Grade Calibration Weight, 5g |
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Accuris Aspire Laboratory Aspirator Set - EACH |
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| ASP1086 | Scientific Laboratory Supplies | EACH | EUR 1906.2 |
Filter for Accuris Microplate Reader, 340 nm |
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| MR9600-340 | Benchmark Scientific | 1 PC | EUR 309.52 |
Filter for Accuris Microplate Reader, 370 nm |
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| MR9600-370 | Benchmark Scientific | 1 each | EUR 309.52 |
Filter for Accuris Microplate Reader, 380 nm |
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| MR9600-380 | Benchmark Scientific | 1 PC | EUR 309.52 |
Filter for Accuris Microplate Reader, 405 nm |
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| MR9600-405 | Benchmark Scientific | 1 PC | EUR 232.68 |
Filter for Accuris Microplate Reader, 415 nm |
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| MR9600-415 | Benchmark Scientific | 1 PC | EUR 232.68 |
Filter for Accuris Microplate Reader, 420 nm |
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| MR9600-420 | Benchmark Scientific | 1 PC | EUR 232.68 |
Filter for Accuris Microplate Reader, 450 nm |
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| MR9600-450 | Benchmark Scientific | 1 PC | EUR 232.68 |
Filter for Accuris Microplate Reader, 470 nm |
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| MR9600-470 | Benchmark Scientific | 1 PC | EUR 232.68 |
Filter for Accuris Microplate Reader, 492 nm |
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| MR9600-492 | Benchmark Scientific | 1 PC | EUR 232.68 |
Filter for Accuris Microplate Reader, 510 nm |
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| MR9600-510 | Benchmark Scientific | 1 PC | EUR 232.68 |
Filter for Accuris Microplate Reader, 520 nm |
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| MR9600-520 | Benchmark Scientific | 1 PC | EUR 232.68 |
Filter for Accuris Microplate Reader, 532 nm |
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| MR9600-532 | Benchmark Scientific | 1 PC | EUR 232.68 |
Filter for Accuris Microplate Reader, 540 nm |
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| MR9600-540 | Benchmark Scientific | 1 PC | EUR 232.68 |
Filter for Accuris Microplate Reader, 546 nm |
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| MR9600-546 | Benchmark Scientific | 1 PC | EUR 232.68 |
Filter for Accuris Microplate Reader, 560 nm |
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| MR9600-560 | Benchmark Scientific | 1 PC | EUR 232.68 |
Filter for Accuris Microplate Reader, 562 nm |
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| MR9600-562 | Benchmark Scientific | 1 PC | EUR 232.68 |
Filter for Accuris Microplate Reader, 570 nm |
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| MR9600-570 | Benchmark Scientific | 1 PC | EUR 232.68 |
Filter for Accuris Microplate Reader, 578 nm |
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| MR9600-578 | Benchmark Scientific | 1 PC | EUR 232.68 |
Filter for Accuris Microplate Reader, 590 nm |
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| MR9600-590 | Benchmark Scientific | 1 PC | EUR 232.68 |
Filter for Accuris Microplate Reader, 595 nm |
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| MR9600-595 | Benchmark Scientific | 1 PC | EUR 232.68 |
Filter for Accuris Microplate Reader, 600 nm |
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| MR9600-600 | Benchmark Scientific | 1 PC | EUR 232.68 |
Chromosomal abnormalities have been recognized by an optimised bioinformatics pipeline making use of copy quantity variation (CNV) detecting algorithm. On this research, we exhibit a complete workflow masking each wet- and dry-lab procedures supporting a clinically relevant technique for NIPGT-A that may be carried out inside 48 h, which is crucial for the same-cycle blastocyst switch. The described built-in method of non-invasive analysis of embryonic DNA content material of the tradition media can probably complement present pre-implantation genetic screening strategies.