The dUTP/UDG anti-contamination system is a very effective means of controlling the contamination of PCR amplification products. In PCR detection reagents using DNA as a template, it has gradually become a mandatory standard.
However, the commonly used E. coli UDG still has high activity at the usual reverse transcription temperature (42-55℃), which will degrade the cDNA, thus reducing the sensitivity of One Step RT-PCR/qPCR. The Hiscript II U+ One-Step qRT-PCR Probe Kit contains an optimized ratio of dUTP/dNTP mix and heat-labile UDG derived from psychrophilic marine bacteria.
Heat-labile UDG has high activity at room temperature and can completely degrade U-containing double-stranded DNA during the mixing process of the reaction system. When the reaction system is heated to 50-55°C (the optimal HiScript II reaction temperature), the heat-labile UDG is rapidly and completely inactivated, maintaining cDNA integrity and ensuring that detection sensitivity is not compromised. be affected.
Store at -20℃
- Support multiple probe detection;
- UDG anti-contamination system: Heat-labile UDG rapidly degrades U-containing double-stranded DNA contaminants at room temperature. Heat-labile UDG is rapidly and completely inactivated at 50-55°C to maintain cDNA integrity;
- Ultra-high detection sensitivity: single-digit template copies or 0.1 pg of total RNA can be detected
- Ultra-high amplification specificity: AceTaq® DNA polymerase based on a hot chemical start, enzyme activity completely blocked before 95°C, and equipped with a patented specific promoting factor Exactor, which makes the amplification more specific;